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A complete second-order factorial design was used to investigate the effects of temperature (T), incubation time (t) and pathogen-to-phagocyte ratio (R) on in vitro phagocytosis of Glugea caulleryi spores by turbot spleen adherent cells. Two alternative indicators were used to quantify phagocytosis: percentage phagocytosis (PP, i.e. the percentage of cells showing phagocytic activity), and the phagocytic...
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