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We constructed a promoter-probe vector, designated pMX180, carrying a Streptomyces tyrosinase gene as a reporter gene. In the present study, Escherichia coli harboring the plasmid pMX180tac, generated by insertion of a tac promoter into pMX180, produced melanin mediated by tyrosinase, in the presence of antibiotic nucleic acid synthesis inhibitors. This suggests that pMX180tac is useful for visible...
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