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The alcalase-treated casein hydrolysate was separated into positively charged fraction (PCF) and negatively charged fraction (NCF), which were then digested in simulated gastrointestinal juices. RP-HPLC, gel filtration chromatography, and RP-HPLC with pre-column derivatization were respectively used to analyze HPLC chromatogram, molecular weight distribution, and amino acid composition of differently...
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