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A reverse transcription-polymerase chain reaction assay (RT-PCR) is described for the detection of double-stranded RNA (dsRNA) molecules (M1, M2, and L3) associated with La France disease of the cultivated mushroom Agaricus bisporus. RT-PCR was faster and more sensitive than current methods used to detect dsRNA, such as dsRNA extraction and analysis by electrophoresis. Another major advantage of...
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