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Thymic peptide thymosin α 1 (10 -11 to 10 -6 M) is shown to interact with the VIP receptor-effector system in rat and mouse peritoneal macrophages, and both rat peripheral blood lymphocytes and spleen lymphocytes. In all models, thymosin α 1 inhibits 125 I-VIP binding with a potency that is in a range 1000-1700 times lower than that of the native VIP...
In the present study, we examined the effect of pretreatment with VIP and various peptides structurally related to VIP such us PHI, helodermin, and secretin on VIP receptor number and affinity, as well as VIP-stimulated cyclic AMP production in rat peritoneal macrophages. Short-term (5-30 min) exposures of rat peritoneal macrophages to 0.1 μM VIP induced a rapid reduction in specific binding. Pretreatment...
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