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A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25cm 2 cell culture flask (5ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3×10 6 IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The...
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