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A fully automatic single-drop microextraction (SDME) coupled with gas chromatography/mass spectrometry (GC/MS) was developed for the determination of 12 hydroxylated metabolites of polycyclic aromatic hydrocarbons in seawaters. The derivatization reagent was added to the extraction solvent in order to realize one-step extraction and derivatization. High sensitivity of the method could be achieved...
In this study, an aptamer-based fluorescent sensing platform using triple-helix molecular switch (THMS) was developed for the pesticide screening represented by acetamiprid. The THMS was composed of two tailored DNA probes: a label-free central target specific aptamer sequence flanked by two arm segments acting as a recognition probe; a hairpin-shaped structure oligonucleotide serving as a signal...
Rapid mixing of highly viscous solutions is a great challenge, which helps to analyze the reaction kinetics in viscous liquid phase, particularly to discover the folding kinetics of macromolecules under molecular crowding conditions mimicking the conditions inside cells. Here, we demonstrated a novel microfluidic mixer based on Dean flows with three-dimensional (3D) microchannel configuration for...
The direct electron transfer and electrocatalysis of hemoglobin (Hb) immobilized on the phosphonate functionalized multi-walled carbon nanotubes (MWCNTs) are investigated. Fourier transform infrared (FT-IR) spectra, UV–vis spectra and cyclic voltammetry (CV) analyses reveal that the phosphonate functionalized MWCNTs have good biocompatibility for Hb immobilization, and promote the electron communication...
Analysis of fast biochemical reactions requires rapid mixing of solutions. Micromixers can achieve uniform mixing of solutions in a short time and have been recognized as an attractive tool to analyze fast reactions. However, it is still a challenge to design mixers with simple structure and short dead time. Here, a zigzag turbulent micromixer was developed with a rapid mixing time of 16μs at sample...
In this research, a highly sensitive chemiluminescence method based on a sodium hydrosulfite (NaHSO 3 )–hydrogen peroxide (H 2 O 2 ) reaction for the determination of 1-hydroxypyrene (1-OHP) was developed. The response of this system was linear in the range from 0.5 to 50pmol (R 2 =0.9983). The limit of detection for 1-OHP was 100fmol (S/N=3). 1-OHP in airborne particulates...
We have successfully constructed a novel gold film with open interconnected macroporous walls of nanoparticles by combining the hydrogen bubble dynamic template synthesis with galvanic replacement reaction. After modified by a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (MUA), the three-dimensionally (3D) interconnected macroporous Au film has been used as a biocompatible substrate...
A conductive biocomposite film (MWCNTs–PANIFAD) which contains multi-walled carbon nanotubes (MWCNTs) along with the incorporation of poly(aniline) and poly(flavin adenine dinucleotide) co-polymer (PANIFAD) has been synthesized on gold and screen printed carbon electrodes by potentiostatic methods. The presence of MWCNTs in the MWCNTs–PANIFAD biocomposite film enhances the surface coverage concentration...
Determination of brominated flame retardants in electrical and electronic equipments (EEE) was achieved through microwave-assisted extraction (MAE) and gas chromatography–mass spectrometry. Polybrominated biphenyls (PBBs) and polybrominated diphenyl ethers (PBDEs) including mono-brominated through deca-brominated congeners were qualified and quantified with good linearity (0.9963–0.9998) and repeatability...
By coupling flow-injection with laser-induced fluorescence detection, a setup was developed and a novel method combining fluorescence resonance energy transfer (FRET) and flow-injection analysis (FIA) was proposed for the determination of vitamin B 12 (VB 12 ) based on its fluorescence quenching on the system of acridine orange (AO)/rhodamine 6G (R6G). The effective energy transfer...
A novel flow injection analysis (FIA) method with Rayleigh light scattering (RLS) detection was developed for the determination of protein concentrations. This method is based on the weak intensity of RLS of p-nitrohenzene-azo-3,6 disulfo-1-amino-8-naphthol-7-azo-benzene disodium salt (Amide Black-10B) which can be enhanced by addition of protein in weakly acidic solution. It has proved that the application...
Glutathione peroxidase (GPX) is an important antioxidant enzyme, which plays an important role in scavenging reactive oxygen species. To obtain humanized GPX catalytic antibodies, the phage displayed human antibody library on the surface of the filamentous bacteriophage was used to select novel antibodies by repetitive screening. Phage antibodies B8, H6 and C1 with the GSH-binding site were obtained...
A method for the determination of the earthy and musty odors geosmin, 2-methylisoborneol (2-MIB), 2-isobutyl-3-methoxy pyrazine (IBMP), 2-isopropyl-3-methoxy pyrazine (IPMP) and 2,4,6-trichloroanisole (2,4,6-TCA) in water by headspace solid-phase microextraction (HSSPME) combined with gas chromatography–ion trap mass spectrometry (GC–ITMS) is described. Several parameters of the extraction and desorption...
In this paper, electroanalytical method of tetrakis (4-carboxylphenyl) porphyrin (TCPP) has been established. In a supporting electrolyte of KH 2 PO 4 -Na 2 HPO 4 (pH 7.0), a sensitive second derivative reduction peak of TCPP was found by single-sweep oscillopolarography. The potential peak is -0.70V (versus SCE).The relationship between peak height and the concentration...
The new reagent N-(m-Methylphenyl)-N'-(sodium p-aminobenzenesulfonate)-thiourea (MMPT) was synthesized and its structure was confirmed by elemental analysis, IR, UV and 1 HNMR spectra. The apparent molar absorptivities of usual ions were determined and the chromogenic reaction with microamounts of platinum(IV) was studied in detail. In the medium of an HAc-NaAc buffer solution, MMPT can react...
The interaction between a typical derivative of artemisinin and hemin was investigated by electrochemical and spectroelectrochemical methods. This derivative can be reduced via hemin-catalysis at the glassy carbon electrode, the cathodic overpotential is decreased by ca. 650 mV. A HPLC method for separating the products of the catalytic reaction was established. They were identified either in H ...
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