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Whey powder, a by-product of dairy industry, is an attractive raw material for value-added products. In this study, utilization of whey powder as substrate for low-cost preparation of β-galactosidase as main product and ethanol as by-product were investigated by a litre-scale integrated strategy, encompassing fermentation, isolation, permeabilization and spray drying. Firstly, through development...
A novel integrated process was developed successfully for co-production of β-galactosidase and ethanol using lactose as substrate, containing fermentation (Kluyveromyces lactis), isolation, permeabilization (a new recycling process) and spray drying. Firstly, a new fed-batch strategy optimized co-produced β-galactosidase at 105.91U/mL and ethanol at 32.16mg/mL, 4.40-fold and 10.82-fold increase over...
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