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Studies on the activity of Complex I from Escherichia coli in the presence of different metal cations revealed at least two high affinity metal-binding sites. Membrane-bound or isolated Complex I was activated by K + (apparent binding constant ∼125 μM) and inhibited by La 3+ (IC 50 = 1 μM). K + and La 3+ do not occupy the same site. Possible localization of...
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