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The enantioselective hydrolysis of (R,S)‐2,3‐diphenylpropionic methyl ester ((R,S)‐2,3‐2‐PPAME) catalyzed by lipase to (R)‐2,3‐diphenylpropionic acid ((R)‐2,3‐2‐PPA) was studied in an aqueous system. The catalytic effects of different types of lipase were compared, and Candida antarctica lipase A (CALA) with higher catalytic activity and enantioselectivity was selected. Hydroxypropyl‐β‐cyclodextrin...
Recycling high‐speed counter‐current chromatography was successfully applied to the preparative separation of oxybutynin enantiomers. The two‐phase solvent system consisted of n‐hexane, methyl tert‐butyl ether, and 0.1 mol/L phosphate buffer solution (pH = 5.0) with the volume ratio of 6:4:10. Hydroxypropyl‐β‐cyclodextrin was employed as the chiral selector. The influence of factors on the chiral...
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