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Since bovine in vitro fertilization became possible in the early 80s, a lot of effort has been done to clarify the mechanisms of what seems more and more one of the crucial steps in this procedure, being oocyte maturation. Undoubtedly, many biological factors act together to prepare the immature oocyte for a successful development to a competent embryo after fertilization. Defects in oocyte maturation...
Relatively poor viability of in vitro-produced embryos may be caused by inadequate culture conditions for the oocytes and/or the embryos. This research was to compare the ultrastructural differences between oocytes of various stages collected during maturation in vitro vs. in vivo. In vivo matured oocytes were collected from live superovulated cows or adult heifers by transvaginal ultrasound-guided...
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