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We have developed a magnetic enzyme-linked immunosorbent assay (MELISA) based poly-L-lysine (PLL) mediated brushes on magnetic nanoparticles (MNPs) for detection of Escherichia coli O157:H7 in river water samples. In the MELISA, we couple PLL on the surface of magnetic nanoparticles to prepare the PLL brushes (MPLLBs). PLL with plentiful amine groups provides multi-binding sites to allow the binding...
A chemiluminescent method for nitrite detection was developed on microfluidic chip. Carbon dots-NaNO2− acidified H2O2 system was adopted. Chemiluminescence (CL) spectrum of this system was detected. The radiative recombination of hole-injected and electron-injected carbon dots explained their CL property. Spiral microchannels were designed on the microfluidic chip to allow enough reaction time for...
In this work, a simple and convenient method for the detection of mini-short tandem repeat (miniSTR) loci has been developed by the combination of polymerase chain reaction (PCR) and microchip electrophoresis (MCE). Degraded or inhibitor DNA greatly limited STR loci analysis. Therefore, The proper primers was designed as close as possible to the STRs region to produce smaller size STRs, and made the...
The peroxidase activities of the complexes of hemin and intermolecular four-stranded G-quadruplexes formed by short-stranded X n G m X p sequences (X=A, T or C), especially T n G m T p sequences, were compared. The results, combining with those of circular dichroism (CD) spectra and acid–base transition study for DNA–hemin complexes, provide some important...
Multifunctional nanoparticles possessing magnetic, long-lived fluorescence and bio-affinity properties have been prepared by copolymerization of a conjugate of (3-aminopropyl)triethoxysilane bound to a fluorescent Eu 3+ complex, 4,4′-bis(1″,1″,1″-trifluoro- 2″,4″-butanedion-4″-yl)chlorosulfo-o-terphenyl-Eu 3+ (APS-BTBCT-Eu 3+ ), free (3-aminopropyl)triethoxysilane (APS) and...
C/Fe nanocomposite (CFN) was synthesized by a procedure similar to an exfoliation/adsorption process to intercalate Fe 3+ into graphite oxide (GO) layers and would be reduced in a H 2 atmosphere. The results of X-ray diffractometry (XRD) and transimission electron microscopy (TEM) show that the form of CFN is carbon nanotube–Fe nanoparticle composite with α-Fe distributed on the nanotube...
Catechins are strong autoxidant to produce steady intermediate in alkali solution. In present work, we, for the first time, developed a electrochemical method based on ruthenium tris (2, 2′) bipyridyl (Ru(bpy) 33+ ) modified boron-doped diamond (BDD) electrode to investigate the electrochemical reduction of catechin autoxidation intermediate with its existence verified by the electron...
A new electrochemical immunoassay strategy was proposed based on electrostatic interaction of natural polymers. The chitosan-entrapped carbon paste electrode (CCPE) was used as the base electrode, alginates as carriers for the reactants and horseradish peroxidase (HRP) as enzyme label in the immunoassay. The immunoassay was firstly carried out using the homogeneous competitive immune format and a...
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