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By designing and coupling a functional peptide, Gly-Leu-Ala-Cys-Ser-Gly-Phe-Pro-Arg-Gly-Arg-Trp, which could be cleaved by thrombin at the site of Arg-Gly (R-G), to the surface of gold nanoparticles (Au-NPs), we propose a simple spectrofluorometry for thrombin (TRB) in this contribution. Experiments showed that the peptide coupled to the surface of Au-NPs in a Tris–HCl buffer at 37°C could be cleaved,...
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