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Ellagitannins are the most abundant polyphenols in pomegranate (Punica granatum) husk and contribute greatly towards its biological properties. A pre-enriched pomegranate husk powder was extracted with water and then further purified by an Amberlite XAD-16 column. Punicalagin (PC) anomers were eluted using a gradient of methanol and water. Fractions eluted with 20% and 25% methanol yielded 1.08g of...
We investigated the effect of punicalagin (PC) on benzo[a]pyrene (BP)-induced DNA adducts in vitro and in vivo. Incubation of BP (1μM) with rat liver microsomes, appropriate co-factors and DNA in the presence of vehicle or punicalagin (1–40μM) showed dose-dependent inhibition of the resultant DNA adducts, with essentially complete (97%) inhibition at 40μM. However, PC failed to inhibit anti-BPDE-induced...
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