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This study focused on cloning human interleukin-26 (hIL-26) and expressing it in Escherichia coli (E. coli) efficiently. Two pairs of primers were synthesized in accordance with the hIL-26 gene reported by GenBank. The hIL-26 gene was cloned by nest PCR following the first round RT-PCR from human peripheral blood monocytes total RNA, and then the PCR product was cloned into pMD18-T vector. Colony...
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