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We study the phase behavior when a mixture of two semi-flexible polymers, giant DNA and alginate, is highly confined mixture in a cell-sized scale space. The characteristic lengths of giant DNA is different from those of alginate. Through the microscopic observation, we found that the volume/surface area ration and the higher-order structure of giant semi-flexible polymer are the crucial determinants...
In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with...
This paper presents the evaluation of ultra-minimal spout amount from micro-nano pipettes into phospholipid-coated micro-droplets. The pipettes can be used to control the local environment around/inside single cells. Conventionally, the microinjection with pipette has been conducted by air pressures. In this method, the spout of high viscosity solutions is difficult because of the frictional forces...
Detection of change in microenvironmental pH may be essential for analyzing biological processes, if transitory biodegradable scaffold materials are used for tissue engineering in bone regeneration. In this study, we created a pH indicator immobilized-gel-sheet for pH measurement. By using gel-sheet, we can investigate the local microenvironment of biodegradable materials, such as octacalcium phosphate...
We propose a nano size clock, capsulated into micro size liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ??24-hour (circadian) period. In cyanobacteria, circadian clock was reconstituted by only three proteins. We reconstitute these proteins and adenosine...
Single cell analysis has attracted much attention to reveal the detailed and localized biological information. Local environmental control technique is desired to analyze the detailed and localized properties of a single cell. In this paper, we propose the local environmental control system with nano/micro dual pipettes to control the local reagent concentration dynamically and arbitrarily. Local...
For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture using the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of E. coli gcl promoter strength.
In this study, we tried to create a polydimethyl -siloxane (PDMS) scaffold with controlled pore configurations to investigate the proliferation and differentiation of the cells seeded 3-dimensionally. The three-dimensional scaffold was designed to investigate the effect of mechanical stress to the cells by utilizing the intrinsic elasticity of the PDMS. We used a combination of casting mold and lamination...
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