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Förster resonance energy transfer (FRET) microscopy is an important tool suitable for studying molecular interactions in living cells. Optical section structured illumination microscopy (OS‐SIM), like confocal microscopy, has about 200 nm spatial resolution. In this report, we performed quantitative 3‐cube FRET imaging in OS‐SIM mode and widefield microscopy (WF) mode, respectively, for living cells...
Three‐cube Förster resonance energy transfer (FRET) method is the most extensively applied approach for live‐cell FRET quantification. Reliable measurements of calibration factors are crucial for quantitative FRET measurement. We here proposed a modified TA‐G method (termed as mTA‐G) to simultaneously obtain the FRET‐sensitized quenching transition factor (G) and extinction coefficients ratio (γ)...
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