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Endothelial‐to‐mesenchymal transition is a phenotypic conversion characterized by down‐regulation of vascular endothelial markers and the acquisition of a mesenchymal phenotype. We hypothesized that keloid fibroblasts are of endothelial origin and that endothelial‐to‐mesenchymal transition substantially contributes to collagen accumulation during the development and progression of keloids. Wingless...
We developed a three‐dimensional organotypic multicellular spheroid scar model to mimic the microenvironment of human keloid tissues. Keloid tissues were cultured for 7 days. Changes in total cellularity and apoptotic index in the primary keloid spheroid cultures were evaluated histologically and with a TUNEL assay, respectively. The expression profiles of transforming growth factor‐β (TGF‐β), collagen...
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