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A novel on‐line 2‐D system was developed for peptide and protein mapping. The system combines capillary IEF (cIEF) with pressurized CEC (pCEC) using a micro‐injection valve as the interface. Sample fractions, which were focused and separated in the first‐dimension cIEF based on their differences in pIs, were electrically mobilized and further successively resolved by their differences in size, hydrophobicity,...
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