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Assays of micronucleus are extensively used in genotoxicity testing and in monitoring of human exposure to genotoxic materials. As nuclear buds could be a new source of micronuclei formed in interphase, the assay of contents of nuclear bud in normal and comparison group is needed. In this paper, we proposed a new automatic nuclear buds detection algorithm based on ellipse fitting. Experimental results...
Quantification of DNA damage, which may be caused by radiation or exposure to chemicals, is very important and can be very time consuming and subject to variability if carried out visually. The quantification of scoring DNA damage includes biomarkers such as micronuclei, nucleoplasmic bridges, and nuclear buds as scored in cytokinesis-blocked binucleated cells. In this paper, we present a new algorithm...
Optical cross-correlation methods have been used to study the motion of red blood cells (RBC) in the microcirculation. To evaluate the precision of such a method to determine RBC velocity profiles, we developped a computational model of the microscopy image formation. The following steps were undertaken: (1) a mechanical model was used to mimic three dimensional RBC movements in a tubular parabolic...
Ultrasonic backscattered signals from blood contain frequency-dependent information that can be used to obtain quantitative parameters reflecting the aggregation state of red blood cells (RBCs). Recently, the structure factor size estimator (SFSE) was developed to solve for two parameters: the packing factor W and mean gyration radius of aggregates Rg, expressed in number of RBCs (Yu and Cloutier...
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