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We analyzed intracellular Ca 2+ and cAMP levels in Chinese hamster ovary cells expressing a cloned rat kappa opioid receptor (CHO-κ cells). Although expression of kappa (κ)-opioid receptors was confirmed with a fluorescent dynorphin analog in almost all CHO-κ cells, the κ-specific agonists, U50488H or U69593, induced a Ca 2+ transient only in 35% of the cells. The Ca 2+ response...
We characterized the collagen-induced increase in cytosolic Ca 2+ ([Ca 2+ ] i ) of bovine platelets loaded with the Ca 2+ indicator Fura-PE3/AM. Collagen (10 μg/ml)-induced increase in [Ca 2+ ] i was only partially inhibited by aspirin, a cyclooxygenase inhibitor, or adenosine 3′-phosphate 5′-phosphosulfate (A3P5PS, a P2Y 1 receptor antagonist),...
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