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The partitioning and resolution of two lipases (acidic and neutral) was achieved in an aqueous two-phase system from crude culture supernatant of Bacillus stearothermophilus SB-1. The effects of changes in PEG molecular weight, varying PEG/phosphate system, pH and sodium chloride concentration were examined on the partition coefficient, K in PEG/phosphate system. The best system was PEG 6000 (70%...
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