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The envelope protein of dengue virus is involved in host cell attachment for entry and induction of protective immunity. Current efforts are focused on producing a tetravalent vaccine by mixing four monovalent vaccine components. In this work, we developed a genetic vaccine based on a novel adeno-associated viral (AAV) vector expressing the carboxy-terminal truncated envelope protein (79E) of dengue...
The efficient tetravalent vaccine is urgently needed to provide long-term and all-round immunity against dengue virus. In order to construct the tetravalent attenuated vaccine, the well defined B-cell and T-cell epitopes of the glycoprotein of the DEN-1 HAWAII strain were predicted and analyzed by using network servers. The long RT-PCR was established to amplify the cDNA of DEN-2 NGC strain for constructing...
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