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Applying the genomic library construction strategy and colony screening, a new aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase has been identified, cloned and overexpressed in Escherichia coli, and the enzyme was purified to homogeneity. Kinetic analysis of the AroAP.fluorescens indicated that the full-length enzyme exhibits 10-fold increased IC50 and an approximately 38-fold increased...
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