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Escherichia coli ribonucleotide reductase (RNR) catalyzes the conversion of nucleoside 5′-diphosphates to deoxynucleoside 5′-diphosphates and is a 1:1 complex of two homodimeric subunits: α2 and β2. As a first step towards mapping the subunit interface, β2 (V365C) was labeled with [ 14 C]-benzophenone (BP) iodoacetamide. The resulting [ 14 C]-BP–β2 (V365C) was complexed with α2 and...
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