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Properties of the ascorbate-dependent antioxidative-defence factors from rat liver cytosol (Antonenkov and Sies, 1992, Biol. Chem. Hoppe-Seyler 373, 1111-1116) were investigated. The factors are assayed by their capacity to inhibit Fe/ADP-ascorbate (nonenzymatic) lipid peroxidation. Ammonium sulfate fractionation and gel-filtration of the dialysed cytosol on Sephadex G-200 or Sephacryl S-400 led...
To elucidate a possible role of membrane-bound aldehyde dehydrogenase in the detoxication of aldehydic products of lipd peroxidation, the substrate specificity of the highly purified microsomal enzyme was investigated. The aldehyde dehydrogenase was active with different aliphatic aldehydes including 4-hydroxyalkenals, but did not react with malonic dialdehyde. When Fe/ADP-ascorbate-induced lipid...
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