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A chitosanase-producing bacterium, Bacillus subtilis HD145 CCTCC AB 2010353, was isolated from a soil sample. The gene (csn) encoding chitosanase was cloned, sequenced, and expressed in the Pichia pastoris strain as a soluble and active form. Its expression level could be as high as 800mg/L, and enzymatic activity reached approximately 9000U/mg. The optimum pH and temperature were 5.5 and 50°C, respectively...
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