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Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% identity to qnrS2 genes described in plasmids associated with other Aeromonas species,...
Pathogenic Aeromonas hydrophila Strain AO1 bears a 21kb plasmid encoding several virulence determinants. Infection studies revealed that this isolate induced cytotoxicity in BALB/c mice splenic macrophages involving reactive oxygen species generation. DNA gel, Hoechst 33342, annexin-V and TUNEL assay documented macrophage death induced by 21kb plasmid bearing isolates to be apoptotic in nature. Apoptosis...
Pathogenic isolates of Aeromonas hydrophila obtained from fish affected with ulcerative disease syndrome (UDS) were found to harbor a 21 kb plasmid. These isolates became sensitive to antibiotics to which they were resistant after curing of the plasmid. The cured bacteria did not undergo any change in their biochemical properties but exhibited significant alterations in their structure (SEM), growth...
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