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The objective of this study was to evaluate the effect of teat cleaning by two rolling brushes on oxytocin (OT) release, milk ejection and milking characteristics during milking in a single stall automatic milking system (AMS). Five treatments B0 (no brushing), B1 (one brushing cycle for 16 s, 4 s per teat), B2, B4 and B6 (two, four and six brushing cycles, respectively) were randomly changed and...
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