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Cofactor flavin adenine dinucleotide (FAD) plays a vital role in many FAD‐dependent enzymatic reactions; therefore, how to efficiently accelerate FAD synthesis and regeneration is an important topic in biocatalysis and metabolic engineering. In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α‐keto acid production—from the...
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