Search results for: John E. Pintar

Items from 1 to 20 out of 35 results

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NOP Receptor Antagonists Decrease Alcohol Drinking in the Dark in C57BL/6J Mice

Gloria Brunori, Michelle Weger, Jennifer Schoch, Katarzyna Targowska‐Duda, more

Alcoholism: Clinical and Experimental Research > 43 > 10 > 2167 - 2178

Background The nociceptin/orphanin FQ opioid peptide (NOP) receptor and its endogenous ligand N/OFQ have been implicated in the regulation of drug and alcohol use disorders (AUD). In particular, evidence demonstrated that NOP receptor activation blocks reinforcing and motivating effects of alcohol across a range of behavioral measures, including alcohol intake, conditioned place preference, and vulnerability...

article

Opioids and opioid receptors orchestrate wound repair

Ying Wang, Mihir Gupta, Tasneem Poonawala, Mariya Farooqui, more

Translational Research > 2017 > 185 > C > 13-23

We have previously shown that topical opioids including morphine and its congeners promote healing of full thickness ischemic wounds in rats. We examined the contribution of mu opioid receptor (MOPr)–mediated healing of full thickness ischemic wounds using MOPr and delta or kappa opioid receptor knockout (KO) mice. Wound closure in the early (day 5) as well as later phases was delayed in topical morphine...

article

Mexneurin is a novel precursor of peptides in the central nervous system of rodents

Maura E. Matus‐Ortega, Philippe Leff Gelman, Juan C. Calva‐Nieves, Anabel Flores‐Zamora, more

FEBS Letters > 591 > 12 > 1627 - 1636

Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ‐opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity‐purified rabbit antisera raised against synthetic EMs in a whole‐mouse brain cDNA library. Following this approach, we identify...

chapter

Genetic Studies of Opioid System Function in Mice

Michael A. Ansonoff, Ting Wen, John E. Pintar

The Receptors > The Opiate Receptors > 341-388

Several mechanisms for altering gene expression of opioid system genes have been used to enhance our understanding of this complex system. In particular, production and analysis of classic opioid system knockout (KO) mice deficient in one or more of the four opioid family receptors and precursors encoding respective ligands have been particularly valuable. These strains have been used to examine the...

article

Corrigendum to “The biology of Nociceptin/Orphanin FQ (N/OFQ) related to obesity, stress, anxiety, mood, and drug dependence” [Pharmacology & Therapeutics 141 (2014) 283–299]

Jeffrey M. Witkin, Michael A. Statnick, Linda M. Rorick-Kehn, John E. Pintar, more

Pharmacology & Therapeutics > 2014 > 143 > 3 > 351

article

660 Insulin-Like Growth Factor Binding Protein-4 Is Required for the Growth Effects of Glucagon-Like Peptide-2 in Murine Intestine

Kaori Yamada, Nuvair A. Imam, John E. Pintar, Patricia L. Brubaker

Gastroenterology > 2014 > 146 > 5 Supplement 1 > S-115

article

The biology of Nociceptin/Orphanin FQ (N/OFQ) related to obesity, stress, anxiety, mood, and drug dependence

Jeffrey M. Witkin, Michael A. Statnick, Linda M. Rorick-Kehn, John E. Pintar, more

Pharmacology and Therapeutics > 2014 > 141 > 3 > 283-299

Nociceptin/Orphanin FQ (N/OFQ) is a 17 amino acid peptide that was deorphanized in 1995. The generation of specific agonists, antagonists and receptor deficient mice and rats has enabled progress in elucidating the biological functions of N/OFQ. Additionally, radio-imaging technologies have been advanced for investigation of this system in animals and humans. Together with traditional neurobehavioral...

article

Glucagon-Like Peptide-2 (GLP-2) and Insulin-Like Growth Factor-I (IGF-I) Induce Differential Intestinal Growth in IGF Binding Protein (Igfbp)-3 and -5 Double Knock out Mice

Sangita G. Murali, Patrick Solverson, Wing Pun, Adam S. Brinkman, more

Gastroenterology > 2011 > 140 > 5 Supplement 1 > S-168

article

Potentiating effect of morphine on oral Salmonella enterica serovar Typhimurium infection is μ-opioid receptor-dependent

Jessica M. Breslow, Pu Feng, Joseph J. Meissler, John E. Pintar, more

Microbial Pathogenesis > 2010 > 49 > 6 > 330-335

Previous studies from our laboratory demonstrated that mice treated with morphine pellets are sensitized to Salmonella enterica, serovar Typhimurium infection. However, the opioid receptor antagonist, naltrexone, only partially blocked the effect of morphine, raising the possibility that the opioid might have some of its effects through a nonopioid receptor. To further clarify whether sensitization...

article

The propeptide precursor proSAAS is involved in fetal neuropeptide processing and body weight regulation

Daniel J. Morgan, Suwen Wei, Ivone Gomes, Traci Czyzyk, more

Journal of Neurochemistry > 113 > 5 > 1275 - 1284

J. Neurochem. (2010) 113, 1275–1284. AbstractMice with a targeted mutation in proSAAS have been generated to investigate whether peptides derived from this precursor could function as an inhibitor of prohormone convertase 1/3 (PC1/3) in vivo as well as to determine any alternate roles for proSAAS in nervous and endocrine tissues. Fetal mice lacking proSAAS exhibit complete, adult‐like processing...

article

M1814 Endogenous IGFBP-5 Regulates IGF-I Expression and Intestinal Smooth Muscle Cell Hyperplasia

Sunila Mahavadi, John R. Grider, Karnam S. Murthy, John E. Pintar, more

Gastroenterology > 2010 > 138 > 5 Supplement 1 > S-424

article

Lack of genotype effect on D1, D2 receptors and dopamine transporter binding in triple MOP‐, DOP‐, and KOP‐opioid receptor knockout mice of three different genetic backgrounds

Ji‐hoon Yoo, Alexis Bailey, Micheal Ansonoff, John E. Pintar, more

Synapse > 64 > 7 > 520 - 527

We investigated D1, D2 receptors and dopamine transporter (DAT) binding levels in mice lacking all three opioid receptors and wild‐type (WT) mice on three different genetic backgrounds. Quantitative autoradiography was used to determine the level of radioligand binding to the D1 and D2 receptors and DAT labeled with [³H]SCH23390, [³H]raclopride, and [³H]mazindol, respectively in triple‐opioid receptor...

article

Neuropeptidomic analysis establishes a major role for prohormone convertase‐2 in neuropeptide biosynthesis

Xin Zhang, Hui Pan, Bonnie Peng, Donald F. Steiner, more

Journal of Neurochemistry > 112 > 5 > 1168 - 1179

J. Neurochem. (2010) 112, 1168–1179. AbstractProhormone convertase 2 (PC2) functions in the generation of neuropeptides from their precursors. A quantitative peptidomics approach was used to evaluate the role of PC2 in the processing of peptides in a variety of brain regions. Altogether, 115 neuropeptides or other peptides derived from secretory pathway proteins were identified. These peptides arise...

article

Role of opioid receptor like-1 receptor in modulation of endocrine, immunological, and behavioral responses to the T-cell superantigen staphylococcal enterotoxin A

Elyse M. Mallimo, Mike A. Ansonoff, John E. Pintar, Alexander W. Kusnecov

Journal of Neuroimmunology > 2010 > 218 > 1-2 > 48-56

Opioid receptor like-1 receptor (ORL ₁ ) is selective for orphaninFQ/nociceptin (OFQ/N), a peptide linked to stress. Since immunologic stimuli exert stressor-like effects, the neuroendocrine and behavioral effects of the T-cell superantigen staphylococcal enterotoxin A (SEA) were tested in ORL ₁ ^−/− and ORL ₁ ^+/+ wildtype 129S6 mice. Within 2h of SEA challenge both...

article

Consequences of opioid receptor mutation on actions of univalent and bivalent kappa and delta ligands

Michael A. Ansonoff, Philip S. Portoghese, John E. Pintar

Psychopharmacology > 2010 > 210 > 2 > 161-168

Introduction During the past decade, substantial evidence has documented that opioid receptor heterodimers form in cell lines expressing one or more opioid receptors. More recent studies have begun to investigate whether heterodimer formation also occurs in vivo. Objectives We have used opioid receptor knockout mice to determine whether the in vivo intrathecal (i.t.) pharmacological potency of delta,...

article

The tail pigmentation pattern of C57BL/6J mice affects nociception/pain quantification in the tail flick test

Ting Wen, Michael A. Ansonoff, John E. Pintar

European Journal of Pain > 2009 > 13 > 6 > 564-567

The heat radiant tail flick test is commonly used to quantify nociception and pain levels. Likewise, the C57BL/6J strain of mice is frequently used in pain-related studies as transgenic mice are often backcrossed onto this background. C57BL/6J mice naturally develop non-pigmented patches of variable length on the distal part of the tail that could conceivably modify the response latency in tail flick...

article

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor

Peng Huang, Chongguang Chen, Wei Xu, Su-In Yoon, more

Biochemical and Biophysical Research Communications > 2008 > 365 > 1 > 82-88

The mu opioid receptor (MOR) in the rat and mouse caudate putamen (CPu) and thalamus was demonstrated as diffuse and broad bands by Western blot with a polyclonal antibody against a C-terminal peptide of MOR, which were absent in the cerebellum and brains of MOR-knockout mice. The electrophoretic mobility of MOR differed in the two brain regions with median relative molecular masses (Mr’s) of 75kDa...

article

Differential involvement of endogenous opioids in sucrose consumption and food reinforcement

Michael D. Hayward, Alexandra Schaich-Borg, John E. Pintar, Malcolm J. Low

Pharmacology, Biochemistry and Behavior > 2006 > 85 > 3 > 601-611

Endogenous opioids within the central nervous system are postulated to mediate hedonic aspects of feeding behavior. To identify the relevant endogenous opioid receptor ligands, mice lacking one or two of the opioid peptide families β-endorphin, enkephalins or dynorphins were tested for sucrose preference in a two-bottle free-choice drinking paradigm under drug-naïve conditions and following treatment...

article

Deletion of peptide amidation enzymatic activity leads to edema and embryonic lethality in the mouse

Traci A. Czyzyk, Yun Ning, Ming-Sing Hsu, Bonnie Peng, more

Developmental Biology > 2005 > 287 > 2 > 301-313

Peptidylglycine alpha-amidating monooxygenase (PAM) catalyzes the COOH-terminal amidation of peptide hormones. We previously had found high expression of PAM in several regions of the developing rodent. To determine the function of PAM during mouse embryogenesis, we produced a null mutant of the PAM gene. Homozygous mutants die in utero between e14.5 and e15.5 with severe edema that is likely due...

article

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation

David Kuninger, Ryan Kuzmickas, Bonnie Peng, John E. Pintar, more

Genomics > 2004 > 84 > 5 > 876-889

Peptide growth factors regulate cell fate by activating distinct signal transduction pathways that ultimately influence gene expression. Insulin-like growth factors (IGFs) play central roles in controlling somatic growth and participate in skeletal muscle development and regeneration. In cultured muscle cells, IGF action is critical both for maintaining viability during the transition from proliferating...

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Search results for: John E. Pintar

NOP Receptor Antagonists Decrease Alcohol Drinking in the Dark in C57BL/6J Mice

Opioids and opioid receptors orchestrate wound repair

Mexneurin is a novel precursor of peptides in the central nervous system of rodents

Genetic Studies of Opioid System Function in Mice

Corrigendum to “The biology of Nociceptin/Orphanin FQ (N/OFQ) related to obesity, stress, anxiety, mood, and drug dependence” [Pharmacology & Therapeutics 141 (2014) 283–299]

660 Insulin-Like Growth Factor Binding Protein-4 Is Required for the Growth Effects of Glucagon-Like Peptide-2 in Murine Intestine

The biology of Nociceptin/Orphanin FQ (N/OFQ) related to obesity, stress, anxiety, mood, and drug dependence

Glucagon-Like Peptide-2 (GLP-2) and Insulin-Like Growth Factor-I (IGF-I) Induce Differential Intestinal Growth in IGF Binding Protein (Igfbp)-3 and -5 Double Knock out Mice

Potentiating effect of morphine on oral Salmonella enterica serovar Typhimurium infection is μ-opioid receptor-dependent

The propeptide precursor proSAAS is involved in fetal neuropeptide processing and body weight regulation

M1814 Endogenous IGFBP-5 Regulates IGF-I Expression and Intestinal Smooth Muscle Cell Hyperplasia

Lack of genotype effect on D1, D2 receptors and dopamine transporter binding in triple MOP‐, DOP‐, and KOP‐opioid receptor knockout mice of three different genetic backgrounds

Neuropeptidomic analysis establishes a major role for prohormone convertase‐2 in neuropeptide biosynthesis

Role of opioid receptor like-1 receptor in modulation of endocrine, immunological, and behavioral responses to the T-cell superantigen staphylococcal enterotoxin A

Consequences of opioid receptor mutation on actions of univalent and bivalent kappa and delta ligands

The tail pigmentation pattern of C57BL/6J mice affects nociception/pain quantification in the tail flick test

Brain region-specific N-glycosylation and lipid rafts association of the rat mu opioid receptor

Differential involvement of endogenous opioids in sucrose consumption and food reinforcement

Deletion of peptide amidation enzymatic activity leads to edema and embryonic lethality in the mouse

Gene discovery by microarray: identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation

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