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Chinese giant salamander iridovirus (GSIV) is the first known and causative viral pathogen in Andrias davidianus. Developing a sensitive, accurate and specific assay to detect GSIV in samples is essential to prevent the further spread of the pathogen. In this study, we established a droplet digital PCR (ddPCR) assay that targeted the mcp gene of GSIV, enabling rapid and quantitative detection of the...
The cytoplasmic helicases retinoic acid‐inducible gene I (RIG‐I) and melanoma differentiation‐associated protein 5 (MDA5) are two pattern recognition receptor genes belonging to RIG‐I‐like receptor (RLR). They play a crucial role in host sensing of pathogen invasion. Herein, we identified and characterized complete cDNA of RIG‐I and MDA5 in Chinese giant salamander (adRIG‐I and adMDA5). To evaluate...
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