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For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. However, some uncertainties exist concerning the specificity of certain target regions and the sensitivity. To identify the methods which are best suited for diagnostics, 9 single-round and 2 multiple PCR systems...
The gene encoding 34kDa protein gene from Mycobacterium paratuberculosis C-2, chromosomal DNA was amplified by using polymerase chain reaction (PCR), the PCR product was approximately 897bp DNA segment. The PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-34 was constructed successfully. The purified 34kDa protein gene was subcloned into the expression vector pET28a(+), and...
The gene encoding ESAT-6 from Mycobacteriumbovis Vallee111 chromosomal DNA was amplified by using polymerase chain reaction (PCR), the PCR product was approximately 288bp DNA segment. The PCR product was cloned into pGEM-T vector and the cloning plasmid pGEM-T-ESAT-6 was constructed successfully. The purified ESAT-6 gene was subcloned into the expression vector pGEX-4T-3, and the prokaryotic expression...
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