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Adding cholesterol to monolayers of certain phospholipids drives the separation of liquid-ordered from liquid-disordered domains. The ordered phases appear to contain stoichiometric complexes of cholesterol and phospholipid. Furthermore, it has been suggested that the cholesterol in these complexes has a low chemical activity compared to that of the free sterol; i.e., that in excess of the phospholipid...
We probed the kinetics with which cholesterol moves across the human red cell bilayer and exits the membrane using methyl-β-cyclodextrin as an acceptor. The fractional rate of cholesterol transfer (% s−1) was unprecedented, the half-time at 37°C being ∼1s. The kinetics observed under typical conditions were independent of donor concentration and directly proportional to acceptor concentration. The...
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