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We examined the influence of D177N (D178N in humans) mutation on the conformational stability of the S2 region of moPrP C with varying pHs by using the SDSL–ESR technique. The ESR spectrum of D177N at pH 7.5 was narrower than that of Y161R1, referred to as WT ∗ . The ESR spectrum of D177N did not change when pH in the solution decreased to pH 4.0. Our results suggested that the disappearance...
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