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To clarify the role of intestinal microbiota on skatole production and the influences of host gender, individual fecal samples were collected from female, castrated and entire male pigs at the ages of 20, 85, and 185 days. Concentrations of skatole in feces were determined by high performance liquid chromatography (HPLC), and the bacterial community in the pig feces was analyzed using 454-pyrosequencing...
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