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The effects of substrate surface loading rate on biofilm growth and structure were investigated by chemical, biochemical and microscopic methods. Three tubular reactors were operated at equal C:N ratio of 0.1, with substrate loading rates of 1.2, 0.6 and 0.3g-C/m 2 /day. Substrate loading positively influenced the biofilm growth rate. Denser biofilms with lower porosities were formed at higher...
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