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Fully de-N-acetylated chito-oligomers were prepared by de-amination of chitosan using nitrous acid (HNO 2 ). The dimer to hexamer fractions of chitosan oligomers were fractionated with preparative size-exclusion chromatography (SEC) and characterized using 1 H NMR spectroscopy. The purified oligomers were polymerized by reductive N-alkylation by the action of NaCNBH 3 , in...
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