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This article reports the results of a study carried out to evaluate the offline hyphenation of capillary zone electrophoresis with matrix‐assisted lased desorption ionization time of flight mass spectrometry (MALDI‐TOF‐MS) for the analysis of low‐abundant complex samples, represented by the tryptic phosphorylated peptides of phosphoproteins, such as α‐casein, β‐casein, and fetuin. The proposed method...
CE offers the advantage of flexibility and method development options. It excels in the area of separation of ions, chiral, polar and biological compounds (especially proteins and peptides). Masking the active sites on the inner surface of a bare fused silica capillary wall is often necessary for CE separations of basic compounds, proteins and peptides. The use of capillary surface coating is one...
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