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The crystal structures of the chaperonin GroEL Arg13 -> Gly; Ala126 -> Val double mutant, without and in complex with ATPγS, have been determined at atomic resolution. Here, we show that the double mutation Arg13 -> Gly; Ala126 -> Val disrupts negative co-operativity between GroEL rings, with respect to ATP, but has little effect on the positive co-operativity within each ring. Our results...
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