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Ovine microsomal Cytochrome P 450 was used preparatively in vitro to make milligram quantities of Nordiazepam, a known metabolite of the anxiolytic drug Diazepam. The product was purified by reversed phase chromatography and preparative HPLC. This synthetic approach should be of use in the preparation of purified metabolites for analytical reference purposes or research. Using demethylation...
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