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Nitrilase from Rhodococcus ATCC 39484 was found to consist of two species of M r 40 258 +/-2 and 40 388 +/-2 Da. When the enzyme was incubated with nitrile substrates and the reaction quenched with acid, higher M r , species were observed. The mass differences were consistent with addition of a substrate molecule to each species. These results represent the first reported demonstration...
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