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Free‐flow electrophoresis (FFE) has the ability to continuously separate charged solutes from complex biological mixtures. Recently, a free‐flow counterflow gradient focusing mechanism has been introduced to FFE, and it offers the potential for improved resolution and versatility. However, further investigation is needed to understand the solute dispersion at the focal position. Therefore, the goal...
Free‐flow electrophoresis (FFE) enables the continuous separation and collection of charged solutes, and as a result, it has drawn interest as both a preparative and an analytical tool for biological applications. Recently, a free‐flow counterflow gradient focusing (FF‐CGF) mechanism has been proposed with the goal of improving the resolution and versatility of FFE. To realize this potential, the...
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