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Peptides were determined by high-performance liquid chromatography (HPLC) with laser-induced fluorescence detection. Detection was based on pre-column fluorescence derivatization of peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) in acetonitrile (MeCN)-0.1M borate buffer (pH 8.0) at 40°C for 10 min. The peptide derivatives were separated on a reversed-phase column with trifluoroacetic...
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