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Previous studies have demonstrated that germ cells can be derived from mouse embryonic stem cells (ESCs). However, there is still no efficient system, which can visualize the stage of germ cell specification in vitro, and further to identify and enrich germ cells derived from ESCs. Figla (factor in the germline, alpha) gene encodes a germ cell specific transcription factor that coordinates the expression...
In vitro derivation of oocytes from mouse embryonic stem cells (ESCs) would benefit the study of oogenesis. Here, we induced mouse ESCs to differentiate into oocyte-like cells by culturing adherent embryoid bodies (EBs) in different medium, including retinoic acid (RA) and follicular fluid. The extent of differentiation was evaluated through morphology and the expression of certain specific germ cell...
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