Search results for: Wenlu Song

Items from 1 to 20 out of 40 results

article

Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

Wenlu Song, Jun Cheng, Lingkan Ding, Min Liu, more

Environmental Progress & Sustainable Energy > 36 > 5 > 1296 - 1300

Water hyacinth pretreated with microwave‐assisted dilute acid was used for fermentation to produce hydrogen using four kinds of genetically modified bacteria for the first time in this study. The genes of several hydrogenase were over‐expressed in Enterobacter cloacae CICC10017 and Enterobacter aerogenes ATCC13408. Four recombinant strains including E. cloacae/HPP, E. aerogenes/HycE, E. aerogenes/HycG,...

article

Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation

Jun Cheng, Min Liu, Wenlu Song, Lingkan Ding, more

Bioresource Technology > 2017 > 227 > C > 50-55

Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of ⁶⁰Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of...

article

Improvement of fermentative hydrogen production using genetically modified Enterobacter aerogenes

Jinfang Zhao, Wenlu Song, Jun Cheng, Min Liu, more

International Journal of Hydrogen Energy > 2017 > 42 > 6 > 3676-3681

The [NiFe]-hydrogenase 3 of Enterobacter aerogenes contains a large subunit of 60 kDa (HycE) and a small subunit of 30 kDa (HycG). The gene of HycE and the gene of HycG were overexpressed in E. aerogenes separately by using the pGEX-4T-2-cat vector, to obtain two recombinant strains: E. aerogenes/HycE and E. aerogenes/HycG. The hydrogen yields were significantly enhanced by the recombinant strains...

article

Enhancing hydrogen production of Enterobacter aerogenes by heterologous expression of hydrogenase genes originated from Synechocystis sp.

Wenlu Song, Jun Cheng, Jinfang Zhao, Chuanxi Zhang, more

Bioresource Technology > 2016 > 216 > C > 976-980

The hydrogenase genes (hoxEFUYH) of Synechocystis sp. PCC 6803 were cloned and heterologously expressed in Enterobacter aerogenes ATCC13408 for the first time in this study, and the hydrogen yield was significantly enhanced using the recombinant strain. A recombinant plasmid containing the gene in-frame with Glutathione-S-Transferase (GST) gene was transformed into E. aerogenes ATCC13408 to produce...

chapter

Regulator reconstruction strategy of grid-connected inverter in weak grid

Qinglin Zhao, Haopu Zhang, Wenlu Song, Xiaoyi Li, more

2016 IEEE 8th International Power Electronics and Motion Control Conference (IPEMC-ECCE Asia) > 451 - 457

2016 IEEE 8th International Power Electronics and Motion Control Conference (IPEMC 2016 - ECCE Asia)

In order to suppress LCL inherent resonance and grid background harmonic problems, virtual impedance control based on capacitor current feedback and grid voltage feedforward control strategies are typically introduced in grid-connected inverters. However, the inverter control strategy can't make the system operate stably in a weak grid. By analyzing the characteristics of voltage and current in weak...

article

Enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using Enterobacter aerogenes

Richen Lin, Jun Cheng, Lingkan Ding, Wenlu Song, more

Bioresource Technology > 2016 > 207 > C > 213-219

Ferric oxide nanoparticles (FONPs) were used to facilitate dark hydrogen fermentation using Enterobacter aerogenes. The hydrogen yield of glucose increased from 164.5±2.29 to 192.4±1.14mL/g when FONPs concentration increased from 0 to 200mg/L. SEM images of E. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting FONPs served as electron conduits to enhance electron transfer...

article

Sodium borohydride removes aldehyde inhibitors for enhancing biohydrogen fermentation

Richen Lin, Jun Cheng, Lingkan Ding, Wenlu Song, more

Bioresource Technology > 2015 > 197 > C > 323-328

To enhance biohydrogen production from glucose and xylose in the presence of aldehyde inhibitors, reducing agent (i.e., sodium borohydride) was in situ added for effective detoxification. The detoxification efficiencies of furfural (96.7%) and 5-hydroxymethylfurfural (5-HMF, 91.7%) with 30mM NaBH₄ were much higher than those of vanillin (77.3%) and syringaldehyde (69.3%). Biohydrogen fermentation...

article

Inhibitory effects of furan derivatives and phenolic compounds on dark hydrogen fermentation

Richen Lin, Jun Cheng, Lingkan Ding, Wenlu Song, more

Bioresource Technology > 2015 > 196 > C > 250-255

The inhibitory effects of furan derivatives [i.e. furfural and 5-hydroxymethylfurfural (5-HMF)] and phenolic compounds (i.e. vanillin and syringaldehyde) on dark hydrogen fermentation from glucose were comparatively evaluated. Phenolic compounds exhibited stronger inhibition on hydrogen production and glucose consumption than furan derivatives under the same 15mM concentration. Furan derivatives were...

article

Fermentative hydrogen production using algal biomass as feedstock

Ao Xia, Jun Cheng, Wenlu Song, Huibo Su, more

Renewable and Sustainable Energy Reviews > 2015 > 51 > C > 209-230

Hydrogen is considered as an ideal alternative to fossil fuels due to its high energy density by mass and clean combustion product. Using anaerobic bacteria to ferment biomass and produce renewable hydrogen is receiving increased attention. Aquatic algal biomass, which can be sourced from natural algal bloom or mass cultivation, is considered as a promising substrate for hydrogen fermentation. This...

article

Subcritical water hydrolysis of rice straw for reducing sugar production with focus on degradation by-products and kinetic analysis

Richen Lin, Jun Cheng, Lingkan Ding, Wenlu Song, more

Bioresource Technology > 2015 > 186 > Complete > 8-14

The competitive reactions of reducing sugar production and degradation in the subcritical water hydrolysis of rice straw were investigated to optimise reducing sugar yield. The optimised conditions (280°C, 20MPa, rice straw concentration of 5wt.% and agitation speed of 200rpm) resulted in a reducing sugar yield of 0.346g/g rice straw because of the enhanced reducing sugar production and decreased...

article

Characterisation of water hyacinth with microwave-heated alkali pretreatment for enhanced enzymatic digestibility and hydrogen/methane fermentation

Richen Lin, Jun Cheng, Wenlu Song, Lingkan Ding, more

Bioresource Technology > 2015 > 182 > Complete > 1-7

Microwave-heated alkali pretreatment (MAP) was investigated to improve enzymatic digestibility and H₂/CH₄ production from water hyacinth. SEM revealed that MAP deconstructed the lignocellulose matrix and swelled the surfaces of water hyacinth. XRD indicated that MAP decreased the crystallinity index from 16.0 to 13.0 because of cellulose amorphisation. FTIR indicated that MAP effectively destroyed...

article

Fermentative hydrogen and methane cogeneration from cassava residues: Effect of pretreatment on structural characterization and fermentation performance

Jun Cheng, Richen Lin, Lingkan Ding, Wenlu Song, more

Bioresource Technology > 2015 > 179 > C > 407-413

The physicochemical properties of cassava residues subjected to microwave (or steam)-heated acid pretreatment (MHAP or SHAP) were comparatively investigated to improve fermentative hydrogen and methane cogeneration. The hydrogen yield from cassava residues with MHAP and enzymolysis was higher (106.2mL/g TVS) than that with SHAP and enzymolysis (102.1mL/g TVS), whereas the subsequent methane yields...

article

Enhancement of fermentative hydrogen production from hydrolyzed water hyacinth with activated carbon detoxification and bacteria domestication

Jun Cheng, Richen Lin, Wenlu Song, Ao Xia, more

International Journal of Hydrogen Energy > 2015 > 40 > 6 > 2545-2551

To improve fermentative hydrogen production from water hyacinth pretreated with microwave-assisted dilute H ₂ SO ₄ and cellulase, activated carbon (AC) was used to effectively remove fermentative inhibitors in hydrolysates. The removal efficiencies of vanillin, 5-hydroxymethyl furfural and furfural in artificial hydrolysates of water hyacinth were 84.8%, 45.4%, and 39.5%, respectively...

article

Substrate consumption and hydrogen production via co-fermentation of monomers derived from carbohydrates and proteins in biomass wastes

Ao Xia, Jun Cheng, Lingkan Ding, Richen Lin, more

Applied Energy > 2015 > 139 > Complete > 9-16

Fermentative hydrogen production from biomass wastes is a promising technology combining waste treatment and clean fuel production. Biomass wastes have various types and components, while the fundamental fermentation reactions involve monosaccharides and amino acids. In this study, typical monosaccharides (glucose and xylose) and amino acids (glutamic acid, aspartic acid, serine, glycine, arginine...

article

Enhancement of energy production efficiency from mixed biomass of Chlorella pyrenoidosa and cassava starch through combined hydrogen fermentation and methanogenesis

Ao Xia, Jun Cheng, Lingkan Ding, Richen Lin, more

Applied Energy > 2014 > 120 > Complete > 23-30

To enhance energy production efficiency (EPE) from microalgae Chlorella pyrenoidosa (CP), cassava starch (CS) was mixed with CP to optimise the carbon/nitrogen (C/N) ratio and facilitate efficient dark hydrogen fermentation, followed by photo hydrogen fermentation and methanogenesis. Steam heating with dilute acid was a preferred pre-treatment method to hydrolyze mixed biomass. The maximum dark hydrogen...

article

Effects of changes in microbial community on the fermentative production of hydrogen and soluble metabolites from Chlorella pyrenoidosa biomass in semi-continuous operation

Ao Xia, Jun Cheng, Lingkan Ding, Richen Lin, more

Energy > 2014 > 68 > Complete > 982-988

16s rRNA gene sequence analysis is applied in the semi-continuous fermentation of Chlorella pyrenoidosa to monitor changes in the microbial community of HPB (hydrogen-producing bacteria). The microbial community of HPB on the 4th day is mainly composed of one genus (Clostridium) including one primary species (Clostridium butyricum). The microbial community of HPB on the 22nd day is mainly composed...

article

Enhancing enzymatic saccharification of water hyacinth through microwave heating with dilute acid pretreatment for biomass energy utilization

Ao Xia, Jun Cheng, Wenlu Song, Cong Yu, more

Energy > 2013 > 61 > Complete > 158-166

Water hyacinth, as waste biomass, can potentially replace fossil fuels and address pressing issues of energy shortage and serious environmental pollution. In this study, microwave-assisted dilute acid pretreatment was proposed to enhance the enzymatic saccharification of water hyacinth for biomass energy utilization. After the pretreatment, a large amount of hemicellulose in the pretreated water hyacinth...

article

Promotion of H2 production by microwave-assisted treatment of water hyacinth with dilute H2SO4 through combined dark fermentation and photofermentation

Jun Cheng, Ao Xia, Huibo Su, Wenlu Song, more

Energy Conversion and Management > 2013 > 73 > Complete > 329-334

Water hyacinth was treated with microwave-assisted dilute H ₂ SO ₄ to improve saccharification before enzymatic hydrolysis and H ₂ production during dark fermentation. A maximum reducing sugar (RS) yield of 64.4g/100g total volatile solid (TVS) (96.1% of the theoretical RS yield) was achieved when water hyacinth was treated through microwave heating with 1% dilute H ₂ ...

article

Simultaneous determination of bisphenol A, aflatoxin B1, ochratoxin A, and patulin in food matrices by liquid chromatography/mass spectrometry

Wenlu Song, Charlie Li, Bahman Moezzi

Rapid Communications in Mass Spectrometry > 27 > 6 > 671 - 680

RATIONALEBisphenol A has been widely used in plastic containers and this has raised safety concerns for fetuses, infants, and young children. Aflatoxin B1, ochratoxin A, and patulin are among the most toxic regulated mycotoxins found as contaminants in agricultural crops and animal products. To facilitate the analysis of these chemicals for regulatory purposes, we have developed an analytical method...

article

Sequential generation of hydrogen and methane from xylose by two-stage anaerobic fermentation

Jun Cheng, Wenlu Song, Ao Xia, Huibo Su, more

International Journal of Hydrogen Energy > 2012 > 37 > 18 > 13323- 13329

The sequential generation of hydrogen and methane from xylose by two-stage anaerobic fermentation was investigated for the first time in this study. The effects of substrate concentration, bacteria domestication and nitrogen source on hydrogen yield were studied in the first stage. The genetic characterization of the 16S rDNA was used to analyze the flora of strains domesticated with xylose and glucose...

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HYDROGEN (12)
FERMENTATION (8)
HYDROGEN PRODUCTION (8)
METHANE (8)
WATER HYACINTH (7)
HYDROGEN FERMENTATION (6)
ENERGY CONVERSION EFFICIENCY (5)
ENTEROBACTER AEROGENES (4)
CASSAVA STARCH (3)
ENZYMATIC HYDROLYSIS (3)
GLUCOSE (3)
METABOLIC ANALYSIS (3)
MICROWAVE HEATING (3)
DARK AND PHOTO FERMENTATION (2)
DARK- AND PHOTO-FERMENTATION (2)
DETOXIFICATION (2)
HYDROGENASE (2)
METHANOGENESIS (2)
MICROALGAE (2)
RICE STRAW (2)
XYLOSE (2)
AIR DEPOSITION (1)
ALDEHYDES (1)
ALGAE (1)
AMINO ACIDS (1)
ANAEROBIC DIGESTION (1)
ARTHROSPIRA MAXIMA (1)
ARTHROSPIRA PLATENSIS (1)
AUTOFERMENTATION (1)
BACTERIA DOMESTICATION (1)
BACTERIA MUTANT (1)
BIOMASS ENERGY (1)
CASSAVA RESIDUE (1)
CELL IMMOBILIZATION (1)
CELLULOSE (1)
CHINA AND USA (1)
COGENERATION (1)
COMBINED PRODUCTION (1)
CONCENTRATION (1)
CONGENER PROFILES (1)
CYANOBACTERIAL BIOMASS (1)
DARK FERMENTATION (1)
DARK- AND PHOTO- FERMENTATION (1)
DEFATTED MILK POWDER (1)
DOMESTICATION (1)
ENERGY PRODUCTION (1)
ENERGY PRODUCTION EFFICIENCY (1)
ENTEROBACTER CLOACAE CICC10017 (1)
ENZYMOLYSIS (1)
FERRIC OXIDE NANOPARTICLES (1)
FURAN DERIVATIVES (1)
GAS CHROMATOGRAPHY (1)
GRID-CONNECTED INVERTER (1)
H2 PRODUCTION (1)
HETEROFERMENTATION (1)
HETEROLOGOUS EXPRESSION (1)
HOXEFUYH (1)
HUMAN EXPOSURE (1)
HYDROGEN AND METHANE (1)
HYDROGEN-PROMOTING PROTEIN (1)
HYDROLYSIS (1)
IN SITU DEGRADATION (1)
INHIBITION (1)
KINETIC MODEL (1)
LCL FILTER (1)
LEVELS (1)
MICROBIAL COMMUNITY (1)
MICROWAVE (1)
MICROWAVE-HEATED ACID (1)
MICROWAVE-HEATED ALKALI PRETREATMENT (1)
MIXED BACTERIA (1)
NUCLEAR IRRADIATION (1)
OVER EXPRESSION (1)
OVER-EXPRESSION (1)
OVEREXPRESSION (1)
PBDES (1)
PCBS (1)
PHENOLIC COMPOUNDS (1)
PHOTOFERMENTATION (1)
POLYBROMINATED DIPHENYL ETHERS (1)
PREDICTION (1)
PRETREATMENT (1)
QUANTITATIVE STRUCTURE RETENTION RELATIONSHIPS (1)
REDUCING SUGAR (1)
REGULATOR RECONSTRUCTION (1)
RELATIVE RETENTION TIME (1)
RESPONSE SURFACE METHODOLOGY (1)
SEDIMENTS (1)
SEMI-CONTINUOUS FERMENTATION (1)
SODIUM BOROHYDRIDE (1)
SOLUBLE METABOLITES (1)
SOURCE (1)
SOURCE IDENTIFICATION (1)
STATIONARY PHASES (1)
STEAM-HEATED ACID (1)
SUBCRITICAL WATER HYDROLYSIS (1)
SUBSTRATE (1)
THE GREAT LAKES (1)
WEAK GRID (1)
ZERO-POLES' DISTRIBUTION (1)
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International Journal of Hydrogen Energy (14)
Bioresource Technology (9)
Environmental Science & Technology (4)
Applied Energy (2)
Energy (2)
Energy Conversion and Management (1)
Environmental Pollution (1)
Environmental Progress & Sustainable Energy (1)
Industrial & Engineering Chemistry Research (1)
Journal of Chromatography A (1)
Rapid Communications in Mass Spectrometry (1)
Renewable and Sustainable Energy Reviews (1)
Science China Chemistry (1)
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INFONA - science communication portal

Search results for: Wenlu Song

Improving fermentative hydrogen production from water hyacinth with genetically modified bacteria

Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation

Improvement of fermentative hydrogen production using genetically modified Enterobacter aerogenes

Enhancing hydrogen production of Enterobacter aerogenes by heterologous expression of hydrogenase genes originated from Synechocystis sp.

Regulator reconstruction strategy of grid-connected inverter in weak grid

Enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using Enterobacter aerogenes

Sodium borohydride removes aldehyde inhibitors for enhancing biohydrogen fermentation

Inhibitory effects of furan derivatives and phenolic compounds on dark hydrogen fermentation

Fermentative hydrogen production using algal biomass as feedstock

Subcritical water hydrolysis of rice straw for reducing sugar production with focus on degradation by-products and kinetic analysis

Characterisation of water hyacinth with microwave-heated alkali pretreatment for enhanced enzymatic digestibility and hydrogen/methane fermentation

Fermentative hydrogen and methane cogeneration from cassava residues: Effect of pretreatment on structural characterization and fermentation performance

Enhancement of fermentative hydrogen production from hydrolyzed water hyacinth with activated carbon detoxification and bacteria domestication

Substrate consumption and hydrogen production via co-fermentation of monomers derived from carbohydrates and proteins in biomass wastes

Enhancement of energy production efficiency from mixed biomass of Chlorella pyrenoidosa and cassava starch through combined hydrogen fermentation and methanogenesis

Effects of changes in microbial community on the fermentative production of hydrogen and soluble metabolites from Chlorella pyrenoidosa biomass in semi-continuous operation

Enhancing enzymatic saccharification of water hyacinth through microwave heating with dilute acid pretreatment for biomass energy utilization

Promotion of H2 production by microwave-assisted treatment of water hyacinth with dilute H2SO4 through combined dark fermentation and photofermentation

Simultaneous determination of bisphenol A, aflatoxin B1, ochratoxin A, and patulin in food matrices by liquid chromatography/mass spectrometry

Sequential generation of hydrogen and methane from xylose by two-stage anaerobic fermentation

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