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Cell wall bound β-fructosidases (exo-inulinases, EC 3.2.1.80) of the probiotic bacterium Lactobacillus paracasei are the key enzymes involved in fructo-oligosaccharide (FOS) prebiotic fiber utilization. They consist of conservative catalytic region and large variable part with different number of Big3 domains for peptidoglycan binding. In this study, the first overproduction and purification of two...
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