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Cell wall bound β-fructosidases (exo-inulinases, EC 3.2.1.80) of the probiotic bacterium Lactobacillus paracasei are the key enzymes involved in fructo-oligosaccharide (FOS) prebiotic fiber utilization. They consist of conservative catalytic region and large variable part with different number of Big3 domains for peptidoglycan binding. In this study, the first overproduction and purification of two...
The annotation of full genomes of amylolytic lactic acid bacteria (ALAB) leads to conviction that many of them contain a basic pool of chromosomal genes, responsible for starch hydrolysis. However, only the strains that are forced to survive in starchy environment are able to display these genetically determined properties. The aim of the present work is to investigate the genes actually engaged...
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