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A cyanide hydratase from Aspergillus niger K10 was expressed in Escherichia coli and purified. Apart from HCN, it transformed some nitriles, preferentially 2-cyanopyridine and fumaronitrile. V max and K m for HCN were ca. 6.8mmolmin −1 mg −1 protein and 109mM, respectively. V max for fumaronitrile and 2-cyanopyridine was two to three orders of magnitude lower...
This chapter contains sections titled:
Introduction
Distribution and Evolutionary Relationship of Fungal Nitrilases
Structural Properties
Catalytic Properties
Conclusions and Outlook
Acknowledgment
References
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