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Here, a novel microfluidic test kit combining ultrahigh throughput hydrodynamic filtration and sandwich immunoassay is reported. Specifically, nano and microbeads coated with two different, noncompetitive antibodies, are used to capture the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) nucleocapsid (N) proteins simultaneously, forming larger complexes. Microfluidic filtration discards...
COVID‐19 Diagnostics
In article number 2104009, Jiang Xu, Yan Zhang, Taotao Lao, and co‐workers present a handheld microfluidic filtration platform that enables rapid, low‐cost and robust self‐testing of the SARS‐CoV‐2 virus. The cover design emphasizes its important advantages: (1) Equipment‐free handheld injection, represented by the hands manipulation; (2) Visible and easy readout: a Taichi diagram...
Single‐Cell Genome Sequencing
In article number 2100325, Chunyu Li, Bo Ma, and co‐workers present an addressable dynamic droplet array (aDDA) that combines the advantages of static droplet arrays and continuous‐flow droplet platforms. All the sample preparation steps including single‐cell isolation, cell lysis, and amplification are performed in sequence within a sub‐nanoliter (≈300 pL) droplet, resulting...
An addressable dynamic droplet array (aDDA) is presented that combines the advantages of static droplet arrays and continuous‐flow droplet platforms. Modular fabrication is employed to create a self‐contained integrated aDDA. All the sample preparation steps, including single‐cell isolation, cell lysis, amplification, and product retrieval, are performed in sequence within a sub‐nanoliter (≈300 pL)...
Human induced pluripotent stem cells (iPSCs) are ideal for developing personalized medicine. However, the spontaneous differentiation of human iPSCs under conventional 2D and 3D cultures results in significant heterogeneity and compromised quality. Therefore, a method for effectively isolating and expanding high‐quality human iPSCs is critically needed. Here, a biomimetic microencapsulation approach...
Nanotechnology currently enables the fabrication of uniform solid nanoparticles and liquid nano‐emulsions, but not uniform gaseous nanobubbles (NBs). In this article, for the first time, a method based on microfluidics that directly produces monodisperse NBs is reported. Specifically, a two‐component gas mixture of water‐soluble nitrogen and water‐insoluble octafluoropropane as the gas phase are used...
In article number 2001172, Jian Xu, Bo Ma, and co‐workers invent Raman‐activated gravity‐driven single‐cell encapsulation and sequencing (RAGE‐Seq). It produces antimicrobial susceptibility phenotypes and the genome sequence of 99.5% coverage simultaneously from precisely one bacterial cell. The ability to profile and correlate the microbial metabolic phenome and high‐quality genomes at one‐cell resolution...
The small size and low DNA amount of bacterial cells have hindered establishing phenome–genome links in a precisely indexed, one‐cell‐per‐reaction manner. Here, Raman‐Activated Gravity‐driven single‐cell Encapsulation and Sequencing (RAGE‐Seq) is presented, where individual cells are phenotypically screened via single‐cell Raman spectra (SCRS) in an aquatic, vitality‐preserving environment, then the...
Fluidic flow behaviors in microfluidics are dominated by the interfaces created between the fluids and the inner surface walls of microchannels. Microchannel inner surface designs, including the surface chemical modification, and the construction of micro‐/nanostructures, are good examples of manipulating those interfaces between liquids and surfaces through tuning the chemical and physical properties...
The commonly existing cellular heterogeneity plays a critical role in biological processes such as embryonic development, cell differentiation, and disease progress. Single‐cell omics‐based heterogeneous studies have great significance for identifying different cell populations, discovering new cell types, revealing informative cell features, and uncovering significant interrelationships between cells...
Single‐cell analysis offers unprecedented resolution for the investigation of cellular heterogeneity and the capture of rare cells from large populations. Here, described is a simple method named interfacial nanoinjection (INJ), which can miniaturize various single‐cell assays to be performed in nanoliter water‐in‐oil droplets on standard microwell plates. The INJ droplet handler can adjust droplet...
Heterostructure rough spindle‐knot microfibers (HRSFs) are fabricated via a flexible parallel‐nozzle microfluidic method. In this method, the bioinspired HRSF with a roughness gradient between spindle‐knots and joints, can be manufactured in large‐scale, and with which the size of the spindle‐knots and joints can be precisely adjusted by regulating flow rates. The HRSFs, fabricated with chitosan and...
Bacterial biofilms play essential roles in biogeochemical cycling, degradation of environmental pollutants, infection diseases, and maintenance of host health. The lack of quantitative methods for growing and characterizing biofilms remains a major challenge in understanding biofilm development. In this study, a dynamic sessile‐droplet habitat is introduced, a simple method which cultivates biofilms...
This paper introduces super absorbent polymer valves and colorimetric sensing reagents as enabling components of soft, skin‐mounted microfluidic devices designed to capture, store, and chemically analyze sweat released from eccrine glands. The valving technology enables robust means for guiding the flow of sweat from an inlet location into a collection of isolated reservoirs, in a well‐defined sequence...
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